Device

Part:BBa_K2301003:Design

Designed by: Aaron Renberg   Group: iGEM17_Michigan   (2017-10-27)


TlpA36 temperature sensitive repressor system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 105
    Illegal XhoI site found at 977
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1280
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We decided to use sigma70 as out constitutive promoter because it is common and well characterized. We also added the HindIII site to save future teams time and effort having to mutagenize our part to add their own restriction sites.


Source

TlpA36 is a mutant version of a promoter found naturally in Salmonella typhimurium.

References