Device
Part:BBa_K2301003:Design
Designed by: Aaron Renberg Group: iGEM17_Michigan (2017-10-27)
TlpA36 temperature sensitive repressor system
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 105
Illegal XhoI site found at 977 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1280
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We decided to use sigma70 as out constitutive promoter because it is common and well characterized. We also added the HindIII site to save future teams time and effort having to mutagenize our part to add their own restriction sites.
Source
TlpA36 is a mutant version of a promoter found naturally in Salmonella typhimurium.